abstract：:In order to understand further the mechanism of bone fracture repair, and thus to innovate better operative treatment for bone fracture and to design new implant materials for bone repair, microstructures of external periosteal callus (EPC) of repaired femoral fracture in both children and adults were investigated by ...

journal_title：Journal of structural biology

authors： Cui FZ,Wen HB,Su XW,Zhu XD

更新日期：1996-11-01 00:00:00

abstract：:Structural modeling of the HLA-DQ molecules, a group of human histocompatibility antigens linked to autoimmune diseases and immunosuppression-based on the structure of the homologous molecule DR1, has revealed an overall shape typical of the class II histocompatibility molecules, yet with several novel features. These...

journal_title：Journal of structural biology

authors： Paliakasis K,Routsias J,Petratos K,Ouzounis C,Kokkinidis M,Papadopoulos GK

更新日期：1996-09-01 00:00:00

abstract：:Single crystals of UDP-N-acetylglucosamine enolpyruvyltransferase of Enterobacter cloacae have been grown by vapor diffusion using phosphate buffer as the precipitant. The crystals belong to the monoclinic space group C2 with a = 86.9 A, b = 155.9 A, c = 83.8 A, beta = 91.6 degrees. Assuming two monomers per asymmetri...

journal_title：Journal of structural biology

authors： Sack S,Dauter Z,Wanke C,Amrhein N,Mandelkow E,Schönbrunn E

更新日期：1996-07-01 00:00:00

abstract：:Interdoublet sliding rates were assessed in bull sperm, utilizing a freeze-thaw procedure to allow axonemal disintegration. The sliding rate of 23 degrees C increased with increasing MgATP concentrations up to 1 mM ATP, to plateau at 8 microns/sec. The analyzed interdoublet shear in both live and demembranated (Triton...

journal_title：Journal of structural biology

authors： Bird Z,Hard R,Kanous KS,Lindemann CB

更新日期：1996-05-01 00:00:00

abstract：:We have cloned and expressed in Escherichia coli cDNA for rat nuclear transport factor 2 (NTF2), a key cytoplasmic factor that facilitates the import of proteins into the nucleus through nuclear pores. We have used this bacterially expressed material to produce orthorhombic crystals suitable for high-resolution X-ray ...

journal_title：Journal of structural biology

authors： Kent HM,Clarkson WD,Bullock TL,Stewart M

更新日期：1996-03-01 00:00:00

abstract：:MDPP is a fully featured general-purpose image processing package primarily written to support research in structural biology using data gathered by electron microscopy. It has focused on the analysis of images using Fourier techniques, particularly periodic plane-layer and helical structures, but has extensive tools ...

journal_title：Journal of structural biology

authors： Smith PR,Gottesman SM

更新日期：1996-01-01 00:00:00

abstract：:AVS (Application Visualization System) is commercially available software for analyzing and viewing data. AVS is primarily used in the physical sciences and engineering, and here we describe the application of AVS for examining three-dimensional density maps generated by electron microscopy and image processing. For t...

journal_title：Journal of structural biology

authors： Sheehan B,Fuller SD,Pique ME,Yeager M

更新日期：1996-01-01 00:00:00

abstract：:Although the fundamental steps in Fourier-based image analysis of electron micrographs of helical structures have not changed significantly since the techniques were developed 30 years ago, there have been developments which aid both the analysis itself and the interpretation of results. Increases in computational res...

journal_title：Journal of structural biology

authors： Owen CH,Morgan DG,DeRosier DJ

更新日期：1996-01-01 00:00:00

abstract：:The lipopolysaccharide (LPS) of the Salmonella cell surface serves as the receptor for a very large number of bacterial viruses. The tailspike protein from these viruses recognizes the LPS as its initial receptor. It is proposed that the study of the P22 and epsilon 34 tailspike proteins could serve as a model for the...

journal_title：Journal of structural biology

authors： Greenberg M,Dunlap J,Villafane R

更新日期：1995-11-01 00:00:00

abstract：:Zinc-induced sheets of tubulin are two-dimensional crystalline polymers that constitute an ideal sample for high resolution studies of tubulin by electron crystallography. We show that these 2-dimensional tubulin crystals can be stabilized by taxol against low-temperature depolymerization and degradation with time, ea...

journal_title：Journal of structural biology

authors： Nogales E,Wolf SG,Zhang SX,Downing KH

更新日期：1995-09-01 00:00:00

abstract：:We analyzed a tertiary structure of systemin, the first identified polypeptide plant hormone, using two-dimensional NMR spectroscopy. From these data and molecular dynamics calculations we concluded that the peptide can adopt a Z-like-beta-sheet structure, which has previously been found in many specific DNA-binding p...

journal_title：Journal of structural biology

authors： Slósarek G,Kalbitzer HR,Mucha P,Rekowski P,Kupryszewski G,Giel-Pietraszuk M,Szymański M,Barciszewski J

更新日期：1995-07-01 00:00:00

abstract：:The structure of the smooth muscle contractile apparatus was studied using ultrarapid freezing followed by freeze substitution or by longitudinal freeze-fracture, deep-etch, and platinum-carbon replication. Freeze substitution minimises the detrimental effects of chemical fixation and freeze fracture eliminates them e...

journal_title：Journal of structural biology

authors： Hodgkinson JL,Newman TM,Marston SB,Severs NJ

更新日期：1995-03-01 00:00:00

abstract：:A Gatan 1024 x 1024 slow-scan charge-coupled device (CCD) camera has been interfaced to a JEOL4000EX electron cryomicroscope and explored for its usefulness in the electron crystallographic analysis of thin, glucose-embedded crystals of crotoxin complex kept at -125 degrees C. We show that the camera allows for an on-...

journal_title：Journal of structural biology

authors： Brink J,Chiu W

更新日期：1994-07-01 00:00:00

abstract：:The architecture of the native human alpha 2-macroglobulin was studied by cryoelectron microscopy and image processing techniques. The lip, padlock, doughnut, and four-petaled flower views of this homotetrameric proteinase inhibitor were observed in the frozen-hydrated specimen, and a new view, termed eye view, was al...

journal_title：Journal of structural biology

authors： Larquet E,Boisset N,Pochon F,Lamy J

更新日期：1994-07-01 00:00:00

abstract：:Bloodstream forms of Trypanosoma congolense were cocultivated in vitro with vascular endothelial cells. The trypanosomes adhere specifically to the endothelial surfaces of the anterior part of their flagella, as shown by scanning and transmission electron microscopy. The interaction between parasite and host cell is v...

journal_title：Journal of structural biology

authors： Shakibaei M,Milaninezhad M,Risse HJ

更新日期：1994-03-01 00:00:00

abstract：:In this study we investigated the applicability of the (silver-enhanced) immunogold labeling method for atomic force microscopy. Human lymphocytes were labeled with anti-CD3 conjugated to fluorescein isothiocyanate and a secondary antibody (goat anti-mouse) linked with 1- or 30-nm colloidal gold particles. Silver enha...

journal_title：Journal of structural biology

authors： Neagu C,van der Werf KO,Putman CA,Kraan YM,de Grooth BG,van Hulst NF,Greve J

更新日期：1994-01-01 00:00:00

abstract：:The protein tubulin is the main constituent of microtubules. Previous studies have shown that zinc ions induce the formation of crystalline sheets and macrotubes of tubulin. Both crystal types are suitable for structural studies by electron crystallography. However, crystallographic structural analysis of tubulin has ...

journal_title：Journal of structural biology

authors： Wolf SG,Mosser G,Downing KH

更新日期：1993-11-01 00:00:00

abstract：:By direct immunolabeling we have mapped the distribution of photosystem II (PS II) and cytochrome b6/f on the surfaces of photosynthetic membranes isolated from spinach. Photosynthetic membranes were attached to a support and gently disrupted to expose the occluded outer stacked surface, prior to labeling. Polyclonal ...

journal_title：Journal of structural biology

authors： Hinshaw JE,Miller KR

更新日期：1993-07-01 00:00:00

abstract：:Immunoelectron microscopy of serial sections of leaf cells of wheat doubly infected by wheat streak mosaic virus and Agropyron mosaic virus or wheat spindle streak mosaic virus showed that structural proteins or virions associated only with homologous cylindrical inclusions (CI) or with self in aggregates. CI protein ...

journal_title：Journal of structural biology

authors： Langenberg WG

更新日期：1993-05-01 00:00:00

abstract：:Trypsin treatment of staphylococcal alpha-toxin cleaves the molecule into two roughly equally sized parts, which results in inactivation of the toxin. Tetragonal arrays of oligomers, closely resembling the native ones, can however be formed on lipid layers. From tilted views of negatively stained crystals a 3D structu...

journal_title：Journal of structural biology

authors： Olofsson A,Kavéus U,Thelestam M,Hebert H

更新日期：1992-05-01 00:00:00

abstract：:A structural analysis of cells that contained the interferon-alpha-induced lupus inclusions (LI) was performed using a high-voltage electron microscope to determine the exact cellular location of LI and their association with normal cell organelles. LI were induced in the human B lymphoblastoid cell line, Daudi, by cu...

journal_title：Journal of structural biology

authors： Rich SA,Marko M,Gibbons WE

更新日期：1992-01-01 00:00:00

abstract：:The question as to whether keratin intermediate filaments (KIF) are built from a unique "building block" consisting of a pair of coiled-coil molecules has been studied by examining the earliest stages of reassembly of mouse K1/K10 KIF in vitro. Particles formed in protein solutions of about 45 micrograms/ml (near or b...

journal_title：Journal of structural biology

authors： Steinert PM

更新日期：1991-10-01 00:00:00

abstract：:F-plasmid-mediated bacterial conjugation was studied with hfr (traDts) and tra I mutant Escherichia coli donor strains. This allowed us to observe a statistically significant number of conjugation-specific contacts by video and electron microscopy. Single mating events between E. coli were observed in real time by vid...

journal_title：Journal of structural biology

authors： Dürrenberger MB,Villiger W,Bächi T

更新日期：1991-10-01 00:00:00

abstract：:The purified glucocorticoid receptor (GR) from rat liver has been visualised by electron microscopy. The specimens were prepared by spreading on thin carbon support and negatively stained using uranyl acetate. Two forms of GR, the monomeric and the dimeric forms, were identified based on size, chromatographic distribu...

journal_title：Journal of structural biology

authors： Eriksson P,Daneholt B,Wrange O

更新日期：1991-08-01 00:00:00

abstract：:We have studied two members of the family of morphogenetic factors or chaperonins, the GroEL-like factors from Escherichia coli and Bacillus subtilis, in order to determine the possible structural basis of their related function in promoting the correct and efficient assembly of biological oligomers. The main objectiv...

journal_title：Journal of structural biology

authors： Carazo JM,Marco S,Abella G,Carrascosa JL,Secilla JP,Muyal M

更新日期：1991-06-01 00:00:00

abstract：:Gap junctions containing extensive, highly ordered crystalline arrays of hexagonally packed connexons have been isolated from the hepatopancreas of the arthropod, Homarus americanus (American lobster). The structure of such junctions has been studied to a resolution of approximately 25 A in three dimensions by electro...

journal_title：Journal of structural biology

authors： Sikerwar SS,Downing KH,Glaeser RM

更新日期：1991-06-01 00:00:00

abstract：:A novel preparation of spontaneously contracting heart cells embedded in a collagen strand provides an ideal experimental system for correlative structure-function experiments that utilize the techniques of electron microscopy, quantitative electron probe x-ray microanalysis (EPXMA) and imaging. Heart cells grown with...

journal_title：Journal of structural biology

authors： LeFurgey A,Hawkey LA,Ingram P,Lieberman M

更新日期：1991-02-01 00:00:00

abstract：:Human alpha 2-macroglobulin (alpha 2M), a large tetrameric plasma glycoprotein, inhibits a wide spectrum of proteinases by a particular "trapping" mechanism resulting from the proteolysis of peptide bonds at specific "bait" regions. This induces the hydrolysis of four thiol esters triggering both the possible covalent...

journal_title：Journal of structural biology

authors： Boisset N,Taveau JC,Pochon F,Barray M,Delain E,Lamy JN

更新日期：1991-02-01 00:00:00

abstract：:The three-dimensional structure of Nudaurelia capensis beta virus (N beta V) was reconstructed to 3.2-nm resolution from images of frozen-hydrated virions. The distinctly icosahedral capsid (approximately 40-nm diameter) contains 240 copies of a single 61-kDa protein subunit arranged with T = 4 lattice symmetry. The o...

journal_title：Journal of structural biology

authors： Olson NH,Baker TS,Johnson JE,Hendry DA

更新日期：1990-10-01 00:00:00

abstract：:Different types of cells have been imaged with the atomic force microscope. The morphology of the archaebacterium Halobacterium halobium in its dry state was revealed. On a leaf of the small Indian tree Lagerstroemia subcostata a stoma was imaged. The lower side of a water lily leaf was imaged in water showing feature...

journal_title：Journal of structural biology

authors： Butt HJ,Wolff EK,Gould SA,Dixon Northern B,Peterson CM,Hansma PK

更新日期：1990-10-01 00:00:00

abstract：:Molecular genetic studies with prokaryotic microorganisms reveal that many different molecular processes contribute to the formation of spontaneous mutations. Besides infidelities in DNA replication and the consequences of environmental mutagens, enzyme-mediated DNA rearrangements bring about important, evolutionarily...

journal_title：Journal of structural biology

authors： Arber W

更新日期：1990-07-01 00:00:00

abstract：:This article describes the structure and assembly of bacteriophage PRD1, a lipid-containing virus able to infect Escherichia coli. This phage, with an approximate diameter of 65 nm, is composed of an outer protein shell surrounding a lipid-protein membrane which, in turn, encloses the nucleic acid. The phage genome co...

journal_title：Journal of structural biology

authors： Caldentey J,Bamford JK,Bamford DH

更新日期：1990-07-01 00:00:00

abstract：:The morphologic effects of different sulfur-substituted mono- and dicarboxylic fatty acids on rat hepatocytes have been examined. The substance 1,10-biscarboxymethylthiodecane (BCMTD) is blocked for both beta- and omega-oxidation, whereas 1-monocarboxymethylthiodecane (CMTTD) is only non-beta-oxidizable. At equimolar ...

journal_title：Journal of structural biology

authors： Kryvi H,Aarsland A,Berge RK

更新日期：1990-05-01 00:00:00

abstract：:A topological localization of epitopes on the surface of the Aa6 subunit of Androctonus australis hemocyanin has been carried out. First, immunocomplex strings composed of native hemocyanin and monoclonal antibodies were examined in the electron microscope and submitted to an image processing by correspondence analysi...

journal_title：Journal of structural biology

authors： Lamy J,Billiald P,Taveau JC,Boisset N,Motta G,Lamy J

更新日期：1990-03-01 00:00:00